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nitrogen blowdown systems?
Posted: Fri Feb 08, 2008 7:21 pm
by matthew
Hi, this is somewhat related to liquid chromatography, since I'm trying to choose a fraction collector and a nitrogen evaporative system at the same time. I need to choose the right collection format, vial or plate..
I turned a SPE manifold into a n2 system for scintillation vials, but this won't scale up to high throughput... has anyone seen a decent 96-well plate format n2 blow-down system?
Posted: Mon Feb 11, 2008 1:54 pm
by DR
As far as I know, N2 blow-down is used to reduce large fractions (>5mL, for example) to small ones, so it is usually done "off line".
I haven't looked at N2 blow down for about 15 years, but back then, Zymark had a very nice unit that could reduce up to 50mL samples down to nearly nothing in short order 10 or 12 at a time.
If your fractions are smaller, a SpeedVac may be the way to go (chilled centrifuge w/ vacuum applied).
Posted: Mon Feb 11, 2008 5:00 pm
by JGK
Posted: Mon Feb 11, 2008 8:57 pm
by matthew
Thanks DR, JGK.
DR,
My earlier post wasn't very clear, I didn't intend to suggest that the fraction collector would give up plates to the nitrogen generator (although our tecan could probably do something that snazzy).
My speedvac doesn't have a rotor for plates, and I'm not sure that I would use the rotor if we had one... but I do intend to leverage the machine downstream, after I blow off organics (e.g. lyophilize the aqueous fraction). i.e. speedvacs make great boxes.
JGK,
that's a heck of a piece of equipment. Thanks for the find.
Posted: Mon Feb 11, 2008 9:36 pm
by JGK
JGK,
that's a heck of a piece of equipment. Thanks for the find.
I found it but I can't vouch for its effectivness as I've not used one. The manifold I used was an Organomation one
It had 100 positions (10 x 10 grid), waterbath and could accomodate vials or tubes (depending on the rack used . I had no problems when using this model.
Posted: Tue Feb 12, 2008 6:56 am
by Hollow
we got one from this company (not the 96plate version)
http://www.caliperls.com/products/extra ... tation.htm
Posted: Tue Feb 12, 2008 5:50 pm
by matthew
thanks guys. It seems there are a few n2 systems for well plates.
JGK, shat volume and dimension of vials/tubes do you use with that instrument?