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- Posts: 136
- Joined: Mon Nov 19, 2007 3:16 pm
If I have a peak in TIC for morphine dissolved in methanol.
How can I quantitate it in mg%??
How can I quantitate it in mg%??
Simple.... with calibration
Prepare a sample of known concentration in the same matrix, e.g.10mg/mL in methanol. Run your unknown sample and the known sample and compare peak areas.
unknown peak area / known peak area x known concentration = unknown concentration in solution.
That's obviously a single point calibration, if you want more accuracy, run a calibration curve and create a linear regression equation.
If you want a weight% of a solid sample (e.g. drug) take the dilution factor into account when back-calculating.
