by
XL » Wed Jan 23, 2008 6:26 am
Dionex offers the Acclaim Organic Acid column for this application. It is a silica based high-efficiency RP column which is fully compatible with highly aqueous mobile phases. The column housing is non-metallic - PEEK, which would minimizemetal contamination from SST column hardware.
The condition for separating C1 to C7 organic acids:
Column: Acclaim OA, 150x4.0 mm
Mobile Phase: A - MeCN, B - 0.05% phosphoric acid or methanesulfonic acid
Gradient: 0 to 45% A in 11 min and keep for 5 minute (minor modification might be needed)
Flow rate: 1 mL/min
Detection: UV at 210 nm
Here is the typical result you should expect (in Figure 7).
http://www1.dionex.com/en-us/webdocs/25 ... OA_V22.pdf
Due to low wavelength detection, the mobile phase needs to be of carefully prepared with high quality reagents.
If ethylene glycol content is less than 5%, I don't expect too much of trouble. If it does impose some difficulties, using a 250x4.0 mm column should help to some extent.
In case these acids can be analyzed by a RP column due to matrix effect, please let me know so that I can offer an alternative.
Good luck!
