Column Wash and Storage

[Gordo789]

Hi,

I'm pretty new to the forums and to liquid chromatography in general. I'm running a LCMS method on a C18 column and I was wondering if it is ok to wash and store columns in mobile phase containing acid, in this case 0.1% formic acid.

Thanks!

[goxy43]

generally yes. just make sure that there is no salt on the column and that some amount of organic is in the mobile phase you use for storage. 10% acetonitrile with 0.1% FA will do the job.
success

[Victor]

I would not store the column in an acidic solution. The pH of 0.1% formic acid is about 2.7. This can cause slow hydrolysis of the bonded ligands (especially the end-capping) on prlonged storage. I would store the column in 100% acetonitrile. This is completely compatible with your mobile phase, so you can just run your method direct from the storage solvent.

[Jackus]

http://www.sepsci.com/chromforum/viewto ... ght=#34843