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Spiking recovery above 100%...why?
Posted: Mon Jan 14, 2008 7:56 pm
by Anna01
!
A quick question...how is it possible to have recovery consistently between 102-110% when spiking serum samples?
I am spiking with the highest standard. The standards are made in spiked bovine serum albumin PBS buffer...The standard curves are nice and linear.
Just curious...anyone got an idea?
Posted: Mon Jan 14, 2008 8:32 pm
by Uwe Neue
Plasma can both enhance and suppress the response of an analyte. It appears that you caught a case of ion-enhancement...
Posted: Mon Jan 14, 2008 8:43 pm
by Anna01
I can understand that if the internal standard is slightly different. But is it really possible that ionenchasement/suppression can produce 108% recovery when doing isotope dilution ms/ms?
Posted: Tue Jan 15, 2008 1:47 am
by Uwe Neue
Why not? The enhanced or suppressed response can be caused by the mess between MS1 and MS2.
Posted: Tue Jan 15, 2008 8:04 am
by Peter Apps
Is the blank clean - i.e. no peak at the traget retention time ?
Peter