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Protein Binding and Effects of Precipitation

http://www.chromforum.org/viewtopic.php?t=7658

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Protein Binding and Effects of Precipitation

Posted: Thu Jan 10, 2008 7:01 pm
by brewer
I realize that this isn’t really a chromatography question, but…

Can anyone tell me definitively whether or not plasma protein bound small molecules are completely liberated upon precipitation with ACN or MeOH?

Posted: Mon Jan 14, 2008 10:44 am
by HW Mueller
Not necessarily. It is good practice to make sure that these are highly soluble in the extraction solvent, still you have to check the extraction efficiency. We have done this very simply via radioactive standards.
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