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overloading of the column
Posted: Wed Jan 09, 2008 11:58 pm
by olivia
Hi, can anyone tell me what overloading of the column is please
Thanks
Liv
Posted: Thu Jan 10, 2008 1:49 am
by tom jupille
Just what it sounds like: injecting a sample which is so large that the column "can't handle it" and the results are (unfavorably) affected.
You can subdivide into "volume overload" and "mass overload". These again, are just what they sound like: too big a volume (although the mass of analyte is reasonable) and too much analyte mass (although the volume is reasonable).
Posted: Fri Jan 11, 2008 5:05 pm
by LC_labrat
What you will see is a wide poorly shaped peak. Just as Tom said, decreasing your injection volume and/or your concentration should change it to a sharper peak, a more symmetrical and bell shaped peak.
Posted: Fri Feb 01, 2008 10:16 pm
by Jackus
What you will see is a wide poorly shaped peak. Just as Tom said, decreasing your injection volume and/or your concentration should change it to a sharper peak, a more symmetrical and bell shaped peak.
But maybe she is preparative chromatographer. They always work with overloaded columns
