Antioxidant Analysis

[wrighty]

Morning all,

i was wondering if somebody could help me with a problem i've got regarding antioxidant analysis.

we currently add a mixed tocopherol antioxidant to our product and we are looking into analysing the incoming raw material.

the probelm ive got is to how to do this. i original thought about using standarda but these are very pricey and is not cost effective.

i've been looking into this and there is a reference to ISO 9936, could anybody tell me what this is?-

if not does anybody have any suggestions for a quantifying analysis of the raw material then that would be a great help.

cheers

steve

[AA]

http://www.iso.org/iso/iso_catalogue/ca ... mber=38942

ISO 9936:2006 specifies a method for the determination of the contents of free alpha-, beta-, gamma- and delta-tocopherols and tocotrienols in animal and vegetable fats and oils by high-performance liquid chromatography.

For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.

[Bryan Evans]

Tocopherol isomers have been separated on Unison UK-Silica and
Unison UK-Amino:

http://www.silvertonesciences.com/files/TI307E.pdf
http://www.silvertonesciences.com/files/TI166E.pdf