LC-MS assay for Paclitaxel

[Merrin]

Hi,

I was wondering if anyone knows of or could suggest an appropriate LC-MS assay for the chemotherapeutic drug paclitaxel. I have a Shimadzu LCMS QP8000 instrument. Any suggestions will be warmly recieved

Merrin

[JGK]

Seems to be a few out there:

http://www.sigmaaldrich.com/img/assets/3560/T303174.pdf

For instance

[JJG]

This method may be more specific than your needs, but you could likely apply much of the procedure to your application. Here is the journal reference:

Alexander, M.S., Kiser M.M., Culley T., Kern J.R., Dolan J.W., McChesney J.D, Zygmunt J., Bannister S.J. (2002). Measurement of paclitaxel in biological matricies: high-throughput liquid chromatographic-tandem mass spectrometric quantification of paclitaxel and metabolites in human and dog plasma. Journal of Chromatography B (785, 253-261).