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coloumn storage
Posted: Thu Jan 03, 2008 12:24 pm
by dani nurmawan
Hi, i'm new in chromatography.
Can anyone tell me the best practice of coloumn storage? So it will have longer live.
Thanks in advance
Posted: Thu Jan 03, 2008 12:41 pm
by Jackus
First remove buffers and acids, wash with strong solvent, and store.
Washing see
http://www.sepsci.com/chromforum/viewtopic.php?t=7529
Posted: Thu Jan 03, 2008 1:06 pm
by JGK
I think you'll find that most columns come with documentation giving the correct storage conditions for the respective stationary phase.
Failing that, use a generic cleanup and storage procedure as mentioned previousley.
Posted: Thu Jan 03, 2008 2:03 pm
by DR
^^Generally true for RP columns... Extra care to store in a water free appropriate organic solution is good for NP (silica) columns.
I've had problems w/ Waters X-Bridge if not stored in 100% ACN after use.