Chromatography Forum

please explain follwoing what are the thumb rules (basics) for developing method on LC-MS/MS for bioanalytical purpouses?????

1. how to develop a lc-ms/ms method for acidic compounds in human plasma?

A. how to select a buffer for acidc compounds
B. how to select a mobile phase for acidc compounds
C. how to select a Extraction procedure for acidic compounds
D. how to select a column for acidc compounds

2. how to develop a lc-ms/ms method for basic compounds in human plasma?

A. how to select a buffer for basic compounds
B. how to select a mobile phase for basic compounds
C. how to select a Extraction procedure for basic compounds
D. how to select a column for basic compounds

3. how to develop a lc-ms/ms method for neutral compounds in human plasma?

A. how to select a buffer for neutral compounds (zwitter compounds)
B. how to select a mobile phase for neutralcompounds
C. how to select a Extraction procedure for neutral compounds
D. how to select a column for neutral compounds

Dear guru:
I believe that you may want to narrow your question to be eligible for a short answer. Or you may want to attend a seminar by LC Resources to learn.
Method development is a challenge topic, and there are many books written about it. Even if you have a book, you may not understand it all and still you may not be successful because your matrix is an impossible matrix!
Alfred