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How can we select the mobile phase for acidic and basic comp

http://www.chromforum.org/viewtopic.php?t=7578

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How can we select the mobile phase for acidic and basic comp

Posted: Wed Jan 02, 2008 7:51 am
by GURU
How can we select the mobile phase for acidic and basic compounds for bioanalytical methods?

Posted: Wed Jan 02, 2008 7:32 pm
by tom jupille
Most of your questions are not amenable to short answers. All of them are covered in textbooks. I can recommend Practical HPLC Method Development by Syder, Glajch, and Kirkland.

(disclaimer: I have worked with Lloyd Snyder for the past 20 years)

Posted: Mon Jan 07, 2008 2:49 pm
by AdrianF
If you want something free look at http://hplc.chem.shu.edu/HPLC/index.html

the section under pH will be useful. Also study the FAQ section on this site.
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