overloading of the column

[olivia]

Hi, can anyone tell me what overloading of the column is please
Thanks
Liv

[tom jupille]

Just what it sounds like: injecting a sample which is so large that the column "can't handle it" and the results are (unfavorably) affected.

You can subdivide into "volume overload" and "mass overload". These again, are just what they sound like: too big a volume (although the mass of analyte is reasonable) and too much analyte mass (although the volume is reasonable).

[LC_labrat]

What you will see is a wide poorly shaped peak. Just as Tom said, decreasing your injection volume and/or your concentration should change it to a sharper peak, a more symmetrical and bell shaped peak.

[Jackus]

What you will see is a wide poorly shaped peak. Just as Tom said, decreasing your injection volume and/or your concentration should change it to a sharper peak, a more symmetrical and bell shaped peak.

But maybe she is preparative chromatographer. They always work with overloaded columns