Anyone familiar with Nucleosil S3W column use?

[Pet Tan]

Hi All,

Has anyone used Nucleosil S3W before? Can I use polar solvents on it. What are the limitations?

Thanks!

[RWH]

I`m quite sure that there is no such column. Where did You hear of it or where did You get it from?

[Jacinta]

My apologies, I was looking through several different columns and made a mistake. You are right that there's no Nucleosil S3W. The right name of the column is "Spherisorb S3W"

[Uwe Neue]

Waters Spherisorb S3W is a silica column. It can be used in any solvent that you like, except in the alkaline pH in aqueous solvents.

As every silica column, a separation may be sensitive to the water content in your mobile phase, if you use a very nonpolar solvent or solvent mixture such as hexane. If you use polar additives in your mobile phase, the sensitivity to the water content in your mobile phase decreases significantly.