change in elution order
Posted: Mon Dec 17, 2007 6:48 pm
I had a method developed on a chiracil OB-H column with 90% heptane and 10% etoh. I developed a new method for a different compound this time with 90% heptane(0.2%tea) and 10% etoh. I went back to do some runs with the original method 90% heptane and 10% etoh, and the order of the starting material (ketone) and my product (alcohol) is reversed. My alcohol is coming out first and my ketone is coming out second.
Do you think the exposure of TEA to the column is what caused the reverse in elution order, had anyone ever seen this before?
Do you think the exposure of TEA to the column is what caused the reverse in elution order, had anyone ever seen this before?