GPC Calibration

[Jaries]

What are some ways to do internal calibration on Gel Permeation Chromotagrapy? We run narrow molecular weight standards every once in a while to generate a calibration curve but is there a way to do instrumental calibration? Just to make sure the instrument itself is running properly? Would reconditioning the column be a part of it? Any help will be grealty appreciated. Thanks

[Uwe Neue]

If the column plate count does not change and the calibration curve is in order, don't even think about trying to ruin the column by "reconditioning".