1. On what basis buffer pH has to adjust (based pka of drug or based on ka of buffer or both things has to consider) please explain me with better examples?????

2.what i mentioned below for bioanalytical method development predictions were correct or is there any changes. please explain if any tips were there for better clarification????


1.Genaral extraction procedure:-

If a compound is polar solid phase extraction was prefferable
If a compound is non-polar or moderate polar liquid-liquid extraction was prefferable
If a compound is highly protein binding protein ppt was pfrefferable

2.Buffer selection:-

for acidc drugs:- adjust the ph of buffer +/- 1.5 ph units pka of an compound i,e basic ph
for basic drugs:- adjust the ph of buffer +/- 1.5 ph units pka of an compound i,e acidc ph
for acidc drugs:- adjust the ph of buffer +/- 1.5 ph units pka of an compound i,e basic or acidc ph

Column selection:-

for polar drugs :- for acidc drugs C18,C8 and for basic drugs cyanide(CN) columns were prefferable.

mass spectrometric mode selection:-

for acidc drugs -ve mode was prefferable
for basic drugs +ve mode was prefferable

1.when back extraction was prefferable for extraction of drugs from plasma, how back extraction will be done and which type of compounds will be go back extraction ?
2.Whats the difference if carbon load was less or more in column how it will effect on LC if carbon load is less or more?

please explain follwoing questions Elaborately with examples:-

1..What Functional goups will be present if compound is in acidic nature explain this elobaorately?
3.What Functional goups will be present if compound is in basic nature?
4.What Functional goups will be present if compound is in neutral?
5.mechanisum action involved in quadropoles (how ions will separate in quadropoles)