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UPLC for triple quad
Posted: Mon Dec 10, 2007 3:31 pm
by Fluterd
Hello,
We are planning to purchase a LCMSMS(triple quad)-system with UPLC. I'm currently a user of HPLC, so I have no practical experience with UPLC. Now is my question which pressure is required for proper use of UPLC? Is a pump which produces 600 bar enough? Or should we go for 1000 bar?
Thanks a lot!
Posted: Mon Dec 10, 2007 7:37 pm
by JGK
The Waters UPLC we use is rated up to 1500 psi (1000 bar = 14,600 psi)
If you want full use of the UPLC capability you would need the 1000 bar rated pump.
I would also make sure the remainder of your pre-column LC components can take this level of operating pressure.
Posted: Tue Dec 11, 2007 8:39 am
by bert
Also take care that the MS instrument can handle the chromatographic peaks obtained by UPLC.
Posted: Tue Dec 11, 2007 9:12 am
by bartjoosen
To use the benefits of sub 2 µm particles, also keep in mind the dead volume of the system. The Waters UPLC is designed to minimise the dead volume, as this can cause broad peaks.
Also the datapoints/sec which the detector can handle is very important.
If you have a detector which can only scan each second, and you peaks have a peak witdth of only a fraction of a minute, you won't even see your peaks!
Bart
Posted: Thu Dec 13, 2007 11:45 pm
by Kostas Petritis
If you are going to purchase a high end triple quadrupole (latest generations) and plan to analyze < 50 compounds per run in MRM then you should be OK with the UPLC in terms of peak widths and peak detection. If you plan to detect more compounds than that (i.e. if you want to do full scans) a TOF detector might be more suitable...
The Waters UPLC is rated for 15,000 psi (and not 1,500). It would be best that you purchase a UPLC system so that void volumes have been optimized as mentioned by others...