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Anyone familiar with Nucleosil S3W column use?
Posted: Wed Sep 15, 2004 3:50 pm
by Pet Tan
Hi All,
Has anyone used Nucleosil S3W before? Can I use polar solvents on it. What are the limitations?
Thanks!
No such column
Posted: Thu Sep 16, 2004 6:44 am
by RWH
I`m quite sure that there is no such column. Where did You hear of it or where did You get it from?
Anyone familiar with Nucleosil S3W column use?
Posted: Thu Sep 16, 2004 1:23 pm
by Jacinta
My apologies, I was looking through several different columns and made a mistake. You are right that there's no Nucleosil S3W. The right name of the column is "Spherisorb S3W"
Posted: Thu Sep 16, 2004 9:04 pm
by Uwe Neue
Waters Spherisorb S3W is a silica column. It can be used in any solvent that you like, except in the alkaline pH in aqueous solvents.
As every silica column, a separation may be sensitive to the water content in your mobile phase, if you use a very nonpolar solvent or solvent mixture such as hexane. If you use polar additives in your mobile phase, the sensitivity to the water content in your mobile phase decreases significantly.