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Alendronate by LC/MS/MS
Posted: Tue Sep 14, 2004 12:05 pm
by MS_MS
Dear friends
Mostly I found analysis of alendronate using ion-pairing reagent. I want to try for LC-MS/MS, and worried for using ion-pairing reagent. Again, I have to analyzed urine sample. Does anybody have idea for LC-MS/MS analysis for alendronate without using ion-pair reagent. If not exactly same, I will thankful for any comment on ion-pairing reagent using for LC-MS/MS.
Thanks in advance.
Posted: Tue Sep 14, 2004 1:29 pm
by SIELC_Tech
We have the technology which allows to analyze polar compound without ion-pairing reagents. Chehk the following links;
http://allsep.com/brochures/June_2004.pdf
Here is the link to the chemistry behind the Primesep columns:
http://allsep.com/Technology_RetentionO ... pounds.php
And also an application with similar compound -cidofovir (basic function and phosphoric acid residue):
http://allsep.com/makeCmp.php?cmp=Cmp_152
Mobile phase is ACN/water/ Ammonium acetate (or ammonium formate, formic acid, TFA, etc.)
Posted: Tue Sep 14, 2004 10:01 pm
by Einar Ponten
See other discussion;
"HPLC method for Zoledronic acid"
That is also a hydrophilic biphosphonate compound.
Posted: Wed Sep 15, 2004 10:56 pm
by Uwe Neue
We have done similar compounds from plasma and urine by direct injection onto an Oasis mixed mode ion-exchange column directly connected to the MS. Check on our website or call Claude Mallet at Waters.
Posted: Thu Sep 23, 2004 10:24 am
by JNF
I am right now doing alendronate by LC-MS. Just use the USP method and instead of using a RI detector plug in your ms. WARNING: I advise you to use and orthogonal (or Z) source since there will be some deposits from the buffer
It is working fine with me. I have an Agilent MSD machine.
Ciao
JNF