Chromatography Forum

Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/

Appropriate concentrations for DB-5ms columns?!

http://www.chromforum.org/viewtopic.php?t=7326

Page 1 of 1

Appropriate concentrations for DB-5ms columns?!

Posted: Fri Nov 16, 2007 10:04 am
by Roehrs
Hello,

is somebody working with a DB-5ms column (30m, 0.25mm, 0.5um) and has a tip for a appropiate concentration for the samples? Im analysing aromatic compounds (cresol/xylenol/phenol) with toluene as solvent.

Thanks!
Oliver

Posted: Fri Nov 16, 2007 2:57 pm
by chromatographer1
100 mg per liter should be no problem.

Posted: Sat Nov 17, 2007 11:18 am
by Thunderbunny
Depends on your injection parameters - injection size and split ratio. Capacity for this dimension column is approx 200-250ng

Posted: Mon Nov 19, 2007 7:31 am
by Roehrs
I`m injecting 1 ul and using the spitless mode.

Posted: Mon Nov 19, 2007 1:30 pm
by chromatographer1
Oh my, then 100mg/liter is much too concentrated.

Less than 0.1mg per liter is a better working concentration.

best wishes,

Rod
All times are UTC
Page 1 of 1
Powered by phpBB® Forum Software © phpBB Limited
https://www.phpbb.com/