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- Posts: 92
- Joined: Tue Oct 30, 2007 2:17 am
I am trying to develop a method to measure antioxidant leachables. But my drug product (a kind of polar salt) cannot elute out with draft antioxidants HPLC assay.
Could you please help me to have my salt elute out? The following is the comparison of two methods.
Thanks a lot!
Do phenyl columns retain polar chemicals more than ODS columns?
*Drug product assay
Column: Inersil Phenyl 250 mm*4.6 mm*5 um
Mobile phase: 95/5(v/v) pH 3.2 KH2PO4 buffer/ACN
Temperature: Ambient
Injection: 25 uL
Flow: 1.0 mL/min
Detection: 225 nm
Gradient: Isocratic
Run time: 12 min
Elution time (drug product working standard): 7.6 min
*Antioxidants assay
Column: ODS Hypersil 150 mm*4.6 mm*5 mm
Mobile phase: H2O/ACN,
Temperature: 60 °C
Injection: 20 uL
Flow: 1.0 mL/min
Detection: 200 nm
Gradient:
Time %H2O %ACN
0 50 50
11 0 100
25 0 100
30 50 50
35 50 50
Run time: 35 min
Elution time (drug product working standard): Not seen (can not elute out)