Page 1 of 1
HPLC method for Zoledronic acid
Posted: Sat Sep 11, 2004 6:04 am
by N.V.V.S.S. RAMAN
Hai,
Can any body suggest an HPLC method for Zoledronic acid which is a non chromophore compound.
Raman
Posted: Sat Sep 11, 2004 6:08 pm
by AA
Chromatographia
Publisher: Friedrich Vieweg & Sohn-Verlag GmbH & Co.KG
ISSN: 0009-5893 (Paper) 1612-1112 (Online)
DOI: 10.1365/s10337-004-0384-8
Issue: Online First
Analysis of Zoledronic Acid and Its Related Substances by Ion-Pair RP-LC
Y. Jiang1 , X. Q. Zhang1 and Z. R. Xu1
(1) Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road of Shijiazhuang City, China
Received: 5 January 2004 Revised: 4 June 2004 Accepted: 14 June 2004 Published online: 18 August 2004
Posted: Sat Sep 11, 2004 8:52 pm
by Einar Ponten
This is highly hydrophilic biphosphonate compound.
I suggest that you try a ZIC®-HILIC column, using 80% acetonitrile/20% ammonium acetate buffer. Our experience with compounds like ATP are a promising indication for this application as well.
Posted: Sun Sep 12, 2004 2:00 am
by SIELC_Tech
Your compound will retain similar to cidofovir (polar hydrophilic coompound with phosphate group). You can compare it to regular C18 column.
http://allsep.com/makeChr.php?chr=Chr_068
Posted: Sat Sep 25, 2004 10:41 am
by kalyanaraman
Being a hydrophilic bisphosphonate, retention in a reverse phase column will be very difficult. Also peaks may tail because of the Imidazole moiety in the compound. Also the compound will respond only in lower UV region. To solve these issues a quarternary ammonium ion pair (use phosphate or sulphate salts for UV cutoff) mobile phase using a 100% aqeous compatible reverse phase column will help you.