incompatibility of ibuprofen

[paul duque]

hello,friends.

someone knows if ibuprofen molecule has incompatibility with trichloro acetic acid.?

how can I test that question...

Thanks for all

Paul

[AdrianF]

Could you clarify the question? What is the context?

[paul duque]

Could you clarify the question? What is the context?

hello,Mr adrian

I ve some troubles with the quantification of ibuprofen in coated tablets,
I thinked that trichloroacetic acid used in diluting solution cause molecular degradation...

I saw some deformation ,but not always in my interest peak.

thanks for your points of view
Paul

[XL]

Hi,

From the structure of ibuprofen there is no obvious reason to think that it should be unstable in trichloroacetic acid. http://en.wikipedia.org/wiki/Ibuprofen

However, it won't be difficult to test it out. I would do the following:
First, make a standard solution of this compound, say 0.1 mg/mL;
Then, load two auto-sampler vials (1.5 mL) with equal volume (say, 0.95 mL) of above standard solution;
Then, add 0.05 mL of trichloroacetic acid to one vials, and add 0.05 mL of D.I. water to the other vial as the reference;
Finally, inject samples in both vials periodically using the same chromatographic conditions to monitor any change.

Did you have trouble with linearity result? If not, I would look into the sample preparation step. Can you provide more information on the sample prep. procedure, chromatographic conditions, and specifics of the "trouble"?

[AdrianF]

There should be no need to use TCA in the diluent which could cause a disturbance by being at a different pH to the mobile phase. I suggest using the mobile phase for your final dilution.

[Bryan Evans]

Since you are working with finished product, you may want to do a stress study on your API; excipient; and then
API + excipient. Sometimes excipients can degrade under certain conditions (e.g. light, acid, base, temp,ect.).

[paul duque]

thanks a lot for your comments
paul.