Solvent for sample injection in LC-MS/MS

[Spisso]

Hi,
Is it possible to use 0.01M hydrochloric acid as final solvent for sample injection on a ESI LC-MS/MS?
Thanks

[sassman]

The pH of 0.01M HCl is 2. That is about the lower limit that most silica based columns can handle. Polymeric type columns can withstand a lower pH. Since you only inject a small volume (typically 1-50uL), it should not cause much problem with your column or the MS. It might decrease the lifetime of your column slightly, but not much.

[Spisso]

Sassman,
Thank you for your answer.

[jag]

There was one good article published in Elsevier "Importance of injection solution compositin for LC-MS/MS methods" (Journal of Pharmaceutical and Biomedical Analysis 26(2001) 753-767). You may refer this. If you could not get it, provide your mail address, I will mail you when I see your post.

[jbshan]

Dear Jag,
I tried to open the link which you had sent related to the injection solvent in MS. Kindly send the paper to my following mail id: jbshan@gmail.com

thanx
Shan