Diagnosis for system block procedure???
Posted: Wed Oct 24, 2007 2:37 pm
I am a new HPLC user. My Agilent 1100 system was blocked. I listed how to diagnose system block as below. Please add more that I missed and correct any mistake I made.
How to solve frit block problem?
How to do pressure test?
Diagnosis for system block procedure:
1. switch a column to a connecter to check if the pressure can drop. Yes, Column blocked. No, it might not be the column
2. open the connecter to see if there is any liquid coming out (1ml/min). Yes, it is after the column. No, it is before the column
3. Open the black valve. With 1ml/min flow, if the pressure is larger than 5 bar, the frit is blocked (the salt concentration of my mobile phase matters)
4. 6-port valve can be used to diagnose the block location
How to solve frit block problem?
How to do pressure test?
Diagnosis for system block procedure:
1. switch a column to a connecter to check if the pressure can drop. Yes, Column blocked. No, it might not be the column
2. open the connecter to see if there is any liquid coming out (1ml/min). Yes, it is after the column. No, it is before the column
3. Open the black valve. With 1ml/min flow, if the pressure is larger than 5 bar, the frit is blocked (the salt concentration of my mobile phase matters)
4. 6-port valve can be used to diagnose the block location