FAME quantitation
Posted: Thu Oct 11, 2007 2:19 pm
by Wallace
I am new to GC-MS and I need a little help getting started with my project. I am characterizing and quantifying FAMEs extracted from natural products. Does anyone know of the procedure used to quantify the results? I am using a known amount of internal standard (C23:0) in my extractions and I have run the 37 FAME standard mix to determine RT's for FAME's in my extracts. I am unsure of how to relate the integration response for my standard to the other peaks found in my samples? Is there a way to automate the data processing procedure or does each FID data file need to be manually processed? I am using an Agilent 6090N GC with 5973 MS and ChemStation software. Any help or suggestions would be greatly appreciated.
Posted: Fri Oct 12, 2007 2:59 pm
by AICMM
Wallace,
I am sure there are some people who regularly post that can give you better information than this, but I would toss out two things for you to consider. First, in the FAME mix that you already ran, are all the FAMEs at the same level and give you the same response? (I doubt it.) MS is not FID so not a carbon counter per se. If they do, then you have an average response factor that you can then use to quantify unknowns. At that point, I think Agilent can do quantitation of tentatively identified compounds (TIC's) and allow you to use some given response factor (it's been a while.) If I recall correctly, you can click on other components and run quantitation on those clicked components.
(I can get more info on that if you find that will work.)
Second, are you sure other components in the chromatogram are all FAMEs?
Best regards.