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Question about internal standard and its dynamic range

http://www.chromforum.org/viewtopic.php?t=7033

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Question about internal standard and its dynamic range

Posted: Thu Oct 04, 2007 1:36 pm
by jusuom
I would need help in this:

http://www.sepsci.com/chromforum/viewtopic.php?t=7031

Posted: Fri Oct 19, 2007 1:21 pm
by jusuom
Any ideas/remarks from anybody?

Posted: Fri Oct 19, 2007 2:38 pm
by Peter Apps
Intersting points.

You are right about the response for the internal standard needing to be within the dynamic range of the analysis.

The slope and intercepts of the analyte and internal standard calibrations do not need to be similar. The IS does need to be closely similar to the analyte in its behaviour during sample preparation (especially), separation and detection. All else being equal isotope labelled analogues of the analyte are the best internal standards.

Peter
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