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Total Reducible Sugar Analysis in Cane Molasses by GC orHPLC
Posted: Mon Oct 01, 2007 9:14 am
by ATUL MITTAL
Hi Everybody,
i want to analyse total reducible sugar in cane molasses by GC. Please provide me analysis method if anyone is doing this type of analysis.
Thanks in advance to you all.
Posted: Wed Dec 19, 2007 5:50 am
by k.m.seenivasan
hi
i am doing sugar analysis by HPLC.Are you interested in HPLC?Reply
SUgar Analysis in Cane molasses By GC or HPLC
Posted: Wed Dec 19, 2007 9:42 am
by ATUL MITTAL
Thanks for your reply, Please send me the complete method of HPLC so that i can be able to analyse sugars by HPLC. You can send me the method by my e mail also. My e mail ID is
amittal@indiaglycols.net.
Waiting for your reply.
Posted: Wed Dec 19, 2007 11:51 am
by leadazide
Please also post a short describtion of the methode here please..
Posted: Fri Dec 21, 2007 8:50 am
by k.m.seenivasan
Determination of sugars by HPLC.
Detector :Refractive Index Detector
Column :Ion Exchange column( i used Aminex HPX-87P FROM BIORAD)
cOLUMN TEMPERATURE : 80 C
Mobile Phase : Water
Flow : 0.6 ml/min
The column separated Dextrose,fructose and Sucrose.Maltose lies between dextrose and sucrose which we can not separate.So i have selected dextrose,fructose and sucrose.
The instrument used is Agilent 1100 HPLC.
I have tried this in C18 Column but it didn't works.
Kind Regards
Seeni
Posted: Fri Dec 21, 2007 8:57 am
by k.m.seenivasan
If you need the method of extraction of sugars in foods which contains fat and protein?Mail me.I'll send.