Chromatography Forum

Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/

HPLC sample preparation

http://www.chromforum.org/viewtopic.php?t=6999

Page 1 of 1

HPLC sample preparation

Posted: Fri Sep 28, 2007 11:36 am
by vellaikumar
Hi,

I want to know how to prepare std compounds for HPLC in the following concentrations?

100 ppm in 10 ml/1ml
1000ppm in 10 ml

Is there any simple formula to remember this?

Posted: Fri Sep 28, 2007 2:01 pm
by XiZ
1ppm = 0.001 mg/mL ;)

Posted: Tue Oct 02, 2007 8:39 pm
by sassman
>> 1ppm = 0.001 mg/mL

More precisely 1ppm = 1 mg/kg

For samples diluted in water 1ppm = 1mg/L since 1L of water is 1kg

For samples diluted in solvent you need to take the solvents density into account since 1L is not 1kg.
All times are UTC
Page 1 of 1
Powered by phpBB® Forum Software © phpBB Limited
https://www.phpbb.com/