Advertisement
Chromatography Forum

using glycine in mobile phase

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Topic locked

using glycine in mobile phase

avatar
amitavaghosh
generally we are using glycine in mobile phase in related substance method for -zoles(pantoprazole,esomeprazole,lansoprazole,rabeprazole) type compound, what is the reason behind this? what is the role of glycine?

avatar
Jumpshooter
glycine enhances the viscosity of the carrier phase---keeps the 'zoles from having a decreased retention t.
Jumpshooter

avatar
HW Mueller
Jumpshooter, where did you get that?
I have seen glycine used as a buffer and ionic strength modifyer.
Topic locked
3 posts Page 1 of 1
Return to “Liquid Chromatography”

Who is online

In total there are 18 users online :: 1 registered, 0 hidden and 17 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: Amazon [Bot] and 17 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

    Gas Chromatography

      Mass Spectrometry