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EDA.HCl Analysis on Ion-Pair Chromatography
Posted: Wed Sep 26, 2007 5:27 am
by Chandresh K. Soni
Hi to all
My Problem is the analysis of EthylenDiamine Hydrochloride salt on HPLC.
We Use 0.02M Heptane Sulphonic acid Salt as ionpair reagent and found Little response at 200nm But When We Inject HCl the Same R.T. is found . My Querry is That this peak is Realy of EDA.HCl or only due to HCL.
Thanks in Advance for your Valuable Suggestion.
Posted: Wed Sep 26, 2007 6:37 am
by HW Mueller
There should be very little, if any, EDAHCl or HCl in your aqueous solution. You probably see Cl-.
Posted: Wed Sep 26, 2007 3:30 pm
by Chandresh K. Soni
Thanks for Your Valuable Suggestion.
I Think this is Due to n to n* transitions of Free Electron of Chlorine atom. But is there any possibility of Derrivitizaton of EDA. HCl .
Again Thanks
Dr. Chandresh K.Soni
Posted: Wed Sep 26, 2007 8:02 pm
by SIELC_Tech
Ethylenediamine has no UV activity. Whatever you see is base line disturbance caused by injection. You need to use ELSD or derivatize. There are bunch of reagents to derivatize amino groups.
Alternatively you can use ELSD with no ion-pairing reagent in the mobile phase (although I see some people arguing that TFA is IP):
http://www.sielc.com/compound_106.html
http://www.sielc.com/compound_140.html (please note that it is 10 mm column in first application)
Posted: Thu Sep 27, 2007 8:01 am
by HW Mueller
Hm, havn´t seen the n transition terminology used in elementary ions, but the detector doesn´t care anyway.
SIELC_Tec, do you have a UV spectrum of EDA?
Posted: Fri Sep 28, 2007 5:27 pm
by Mark Tracy
If you don't mind getting an ion chromatograph, the Dionex IonPac CS18 column separates ethylenediamine and related substances quite nicely using conductivity detection.
On the other hand, if all you want is assay, perhaps titration or elemental analysis would be enough.