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Determining void time for a Atlantis HILIC column
Posted: Thu Sep 06, 2007 6:47 pm
by mikea
Hi all,
can anyone tell me the proper way to determine the void time on a HILIC column? I assume sodium nitrate would not be the ideal compound to determine this value on a HILIC column.
Thanks
Posted: Thu Sep 06, 2007 7:24 pm
by tom jupille
The easiest way would be to simply inject a blank of ACN/water at a different ratio from your mobile phase, set your detector out at fairly long wavelength and high sensitivity and look for the refractive index shift.
Posted: Thu Sep 06, 2007 8:10 pm
by juddc
That would work, though I might do it a bit differently.
I would think that a mobile phase containing anything from 50-95% ACN and a dilute solution of something like toluene in ACN (maybe 200 ppm?) would do nicely enough.
Posted: Thu Sep 06, 2007 8:46 pm
by Patrik Appelblad
Toluene is what we use for determining the void volume on our Zwitterionic HILIC phases. It works!