Chromatography Forum

I develp a method with r(2) = 0.997. with concentration between 60 to 130 % of working standard.

For routine analysis is enougth to use 1 concentration point ( 2 weights of standard to calculate recovery) or do you recomend 3 concentration level and then extrapolation of areas of unknown

Thanks for your help

Ruben

Dear Ruben,

You are talking about validated method, so if during the routine analyses one meets the system suitability criteria (set during the method development) it is sufficient to do one-point (or single-point) calibration at the working concentration of your analyte.

Best regards