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high molecularm weight molecule analsysis??
Posted: Sat Sep 01, 2007 5:40 am
by rick1112
Hi
I would like to ask which type of columns (Reverse phase) could be used for analysis of a protein which has high molecular weight (in my case 150 K.Da.)..?
Posted: Sat Sep 01, 2007 11:19 pm
by Ruben Ben
Have you tried with HPLC SEC for example columns of Toya Soda TSK Series ?
Good luck
Ruben
Posted: Sun Sep 02, 2007 3:59 pm
by Kostas Petritis
In general C4 or C5 columns with high porosity (4000 A or so). You can also try non-porous or poroshell columns (i.e. non porous center and just 1 um superficial porous media. You can also try the Agilent macropourous column...
Posted: Tue Sep 04, 2007 6:21 pm
by Mark Tracy
You could also use the ProSwift RP-3U monolith columns. See
http://www1.dionex.com/en-us/columns_ac ... 35517.html