Determining void time for a Atlantis HILIC column

[mikea]

Hi all,

can anyone tell me the proper way to determine the void time on a HILIC column? I assume sodium nitrate would not be the ideal compound to determine this value on a HILIC column.

Thanks

[tom jupille]

The easiest way would be to simply inject a blank of ACN/water at a different ratio from your mobile phase, set your detector out at fairly long wavelength and high sensitivity and look for the refractive index shift.

[juddc]

That would work, though I might do it a bit differently.

I would think that a mobile phase containing anything from 50-95% ACN and a dilute solution of something like toluene in ACN (maybe 200 ppm?) would do nicely enough.

[Patrik Appelblad]

Toluene is what we use for determining the void volume on our Zwitterionic HILIC phases. It works!