HPLC

[kathy]

I created an injector program and a sequence. However the injector program wasn't executed when I run the sequence even I called the right method.

Thank you very much in advance for your help.

[k66]

Lets make some test :
Write this program (as example - test program)

1 DRAW (inj. vol.)
2 Inject
3 Wait [calculate wait time = (inj. vol + 5 ul)/flow(ul/min)]
4 valve bypass
5 needle wash in vial 98, 5 times
6 needle wash in vial 99, 5 times
7 needle wash in vial 100, 5 times
8 Needle up
9 Draw 0.0 ul from seat
10 Wait (runtime minus 1 min)
11 Valve mainpass
12 Valve bypass
13 Valve mainpass
14 Valve bypass
15 Valve mainpass

It must works - if not ... will see

[kathy]

I have tried to test this program injector, it was working Ok. However, I don't understand why my injector does not run.

This is my program injector:

1. Draw 2.5 from vial 1
2. Draw 0.5ul from sample

The system executes line 2, but line 1.

Please help me to fix this program injector.

[k66]

I have tried to test this program injector, it was working Ok. However, I don't understand why my injector does not run.

This is my program injector:

1. Draw 2.5 from vial 1
2. Draw 0.5ul from sample

Insert between line 1 and 2 - needle wash

1 Draw 2.5 from vial 1
2 needle wash
3 Draw 0.5ul from sample

or try
1 valve bypass
2 Draw 2.5 from vial 1
3 needle wash
4 Draw 0.5ul from sample
......
x Inject