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non-porous analytical Reverse phase column
Posted: Fri Aug 24, 2007 4:56 am
by rick1112
hi
could any one of you suggest me some non-porous C18 and C4 column..
thanks a lot
Posted: Fri Aug 24, 2007 1:07 pm
by Bryan Evans
Imtakt does have a 2um non-porous C18 column. It's called Presto FT-C18.
Non-porous columns do have their place in the world, but please keep in mind that
retention and loading capacity are significantly less with non-porous as opposed to porous ODS phases.
As for the C4 - are you looking for 300A pore size (i.e. protein separation) or are you looking for a C4 for small molecule application?
Posted: Fri Aug 24, 2007 4:12 pm
by Bart
Hello Rick,
We have a C18 2um non-porous polymeric (PSDVB) column. Please feel free to contact me if you are interested.
Bart Poulsen
Transgenomic, Inc.
Phone: 775-829-7689
email:
bpoulsen@transgenomic.com
Posted: Mon Aug 27, 2007 4:07 am
by rick1112
hi
we are planning to perform RP-HPLC of a large protein it has Mol.Wt. of about 150 K.Da
so could u recomend some columns for this use
Posted: Mon Aug 27, 2007 1:38 pm
by Bryan Evans
Intrada WP-RP (3um) is a wide pore reversed-phase column that was designed for the separation
of highly hydrophobic proteins. Below are proteins ranging in MWT from 670 kDa to 1046 Da:
http://www.imtakt.com/TecInfo/TI289E.pdf
Posted: Mon Aug 27, 2007 8:51 pm
by promochrom
Hi,
HALO column could be a good choice for you. You can find more information from the following website:
http://www.gc-lc.com/Column_Halo.htm
Regards,
PromoChrom