HPLC METHOD FOR IMIDAZOLE

[SRINIVASAN]

Hi to all,


I want devlop a new method for estimation of IMIDAZOLE by Reverse Phase HPLC. Already i have done with YMC ODS (150 x 4.6, 5mm) by using mobile phase 50:50 (acetonitrile : water: 0.1% TEA) . I found the peak is comming before second minute then i change the mobile phase concentration to 20:80(acetonitrile : water) eventhen i did not get any improvement.

Anybody of you can suggest good method for the same. I want retention time about 8.0 minute

Thanks in advance

[Newman768]

Hi SRINIVASAN,

I doupt that you can bring Imidazole on your YMC ODS-A on a retention time of 8 min.
As far as I remember Imidazole is a base. So try a buffered mobile phase (80 to 90% of water) at pH around 8 to 10 with a column like Waters XTerra or Zorbax Extend.
Any form of ion pairing chromatography should work too.
(Guess, the guy from Alltech Sep will post his suggestion in the next time.)

[Uwe Neue]

For such a polar base I always recommend to use HILIC: use a silica column with a mobile phase of about 80% acetonitrile and 20% water. You may need to adjust the pH with a small amount of acetic or formic acid.

[maris]

From my experience, HILIC is much suitable for imidazole and its derivates. Try Si-gel column with water/ACN at low pH.