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Column for pesticide residue
Posted: Tue Aug 21, 2007 8:18 am
by aysal
Dear users
we will determine pesticide residue in food samples by LC-MS/MS. Now, we prepare the site, chemicals, etc. Do you have an idea about the column specification for pesticide studies. One recomendation is Phenomenex Aqua 50 mmx2 mm 5micron 125 A C18. Is there similar kind of that? Is there anyone working on this subject? (pesticides LC-MS/MS)
any idea will be appreciated
Ayhan
Posted: Tue Aug 21, 2007 2:49 pm
by H.Thomas
Your question is a little unspecific. Which pesticides will you analyze? Will it be multiresidue analysis? Then you could take a look at these articles:
http://www.bfr.bund.de/cm/289/fast_mult ... _water.zip
http://www.amchro.com/PDFs/SPE/Quechers-Methode.pdf
An Aqua column should work, though Phenomenex recommends to use Synergi Hydro-RP instead. You might want to use a 125 mm length.
Hartmut
Posted: Tue Aug 21, 2007 3:23 pm
by Bryan Evans
What pesticides will you be working with? If we have the compound name in our database - I can post it on here.
Posted: Wed Aug 22, 2007 11:50 am
by k66
Probably tiabendazole and benomyle (carbendazime)
If yes... simple c18 rp 250 mm x 4,6 should be adequate
If some other please write...
or
Ask the Experts
Posted: Wed Aug 22, 2007 2:41 pm
by aysal
Yes, That's multiresidue method.
Posted: Wed Aug 22, 2007 5:09 pm
by k66
Yes, That's multiresidue method.
so... mayby try Pursuit XRs-C18
look :
http://www.chromatographyonline.com/lcg ... rticle.pdf
What kind pest. you will detected?
Posted: Wed Aug 22, 2007 8:28 pm
by Bryan Evans
Below are chromatograms for thiabendazole and benomyl analyzed on
Cadenza CD-C18 column:
http://www.imtakt.com/TecInfo/TI230E.pdf
http://www.imtakt.com/TecInfo/TI069E.pdf
Re: Column for pesticide residue
Posted: Thu Aug 23, 2007 7:11 am
by k66
Dear users
we will determine pesticide residue in food samples
One more...
If in food matrix - buy some with particle size 5µm
I'm using Waters Atlantic c18 250x4,6 with phenomex guard (for thiabendazole+benomyl as carbendazim + THIOPHANATE-METHYL)
...the tested way