fluctuation in column back pressure

[vijaykhetmalis]

my hplc instrument gives a lot of fluctuation in back pressure, it sometimes increases to high pressure. what could be reason behind this and what is the solution for it?

[DR]

More details please.

If you're running a gradient, this is quite normal. For isocratic runs, this signals a problem with the column or pumping system...(or the plumbing between them).

[Mark Tracy]

Or the detector flow cell

[syx]

I think there is problem with the pump... please inspect the check valves.