Chromatography Forum

Posted: **Sun Aug 12, 2007 4:30 am**

hi
does pH of the sample injected matters(or affect) the stationary phase stability???

Posted: **Sun Aug 12, 2007 6:13 am**

No single answer to that. If the mobile phase is well-buffered and the sample is small and/or dilute, there should be no problems. If your samples are large / high ionic strength / extreme pH, then a guard cartridge would be a good idea.

Posted: **Mon Aug 13, 2007 5:18 am**

hi
i have samples ranging from pH 3 to pH 9, the injection volume for these samples will be 100 microlitmy buffer is ACN+% TFA and water +0.1%TFA??
under these condition can the pH effect stationary phase

Posted: **Mon Aug 13, 2007 4:31 pm**

A guard is probably a good idea for this case, unless the stationary phase is rated for pH 9.

Posted: **Wed Aug 15, 2007 10:27 am**

My guess would be that your biggest problem could be pH incompatibility with resulting bad peak shapes (at the higher sample pH):

Chromatography Forum

pH effect???

pH effect???