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Carotene analysis/quantification
Posted: Fri Aug 10, 2007 8:47 pm
by ntbhat
Hi all:
I am looking to extract carotene from a mix of carotene and FDC blue. I also need to quantify it after extraction. I need to use HPLC for analysis. Any suggestions for HPLC or any other type of analysis will be greatly appreciated.
Posted: Fri Aug 10, 2007 11:40 pm
by Mark Tracy
I have done carotene in a couple matrices:
http://www1.dionex.com/en-us/acclaim_li ... 29720.html shows two, but there are others.
What is your matrix? I would try extraction with ethyl acetate first. Depending on the concentration, you should be able to directly inject the EtOAc extract with very little ill effect.
I have also done FDC dyes, and they are very hydrophilic and won't interfere with carotene. That example is in the data sheet for one of our new detectors:
http://www1.dionex.com/en-us/webdocs/52 ... m%20VI.pdf
Carotene analysis
Posted: Sat Aug 11, 2007 2:18 am
by ntbhat
Thanks Mark Tracy.
The colors are in a polymer matrix. Any suggestions on separation/extraction of carotene from such a matrix?
Posted: Mon Aug 13, 2007 4:54 pm
by Mark Tracy
Tricky. Depends on the polymer. Maybe ASE pressurized extraction. Maybe something else. Try milling it very fine and extracting with warm ethyl acetate. There are a lot of issues with carotenoids analysis; check the archives for previous discussions.