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about mobile buffer concentration

Posted: Wed Aug 08, 2007 7:09 am
by moon704520
hi:
what is the suitable buffer concentration for the comman HPLC analysis?

Posted: Wed Aug 08, 2007 7:54 am
by syx
we use 20 - 25 mM in our lab. it is sufficient and safe from salt-precipitation.

Posted: Wed Aug 08, 2007 8:01 am
by zokitano
Dear moon704520,

Your answer is presented in the topic: Buffers from author: just me,
from 20th October 2005.

You could also search this forum for more interesting threads regarding buffers.

Best wishes

Posted: Wed Aug 08, 2007 11:57 am
by Jackus
Good idea is to use Google. I found for example this page:
http://www.mac-mod.com/tr/pbprep-tr.html