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Carprofen Analysis
Posted: Mon Aug 06, 2007 1:12 pm
by Diego
Dear members of this forum,
I need your help in order to assay the content of carpofen in tablets. I have only C18, CN and Phenyl columns.
Thanks in advance for your help,
Diego
Posted: Mon Aug 06, 2007 1:48 pm
by zokitano
Dear Diego,
Do you need specific method (which includes one of the columns you wrote) for carprofen assaying?
Posted: Mon Aug 06, 2007 4:23 pm
by Mark Tracy
I didn't find anything in USP, but a Google search on "carprofen assay HPLC" turned up this: Journal of Pharmaceutical Sciences (1983) Volume 73, Issue 9 , Pages 1211 - 1215
Posted: Mon Aug 06, 2007 8:30 pm
by Bruce Hamilton
A search of Pubmed should provide some choices, the following was one of 19 hits for Carprofen and HPLC.
Stability-indicating high-performance liquid chromatographic assay method and photostability of carprofen.Wu AB, Chen CY, Chu SD, Tsai YC, Chen FA.
J Chromatogr Sci. 2001 Jan;39(1):7-11.
A rapid, sensitive, and accurate stability-indicating high-performance liquid chromatographic assay method for determining the degradation of carprofen (CPF) is developed and validated under acidic, basic, or photo-irradiated conditions. The analysis is monitored with a Cosmosil 5C18-AR column using a mobile phase of CH3CN-H2O-AcOH (50:49:1, v/v/v) at 260 nm. The developed method satisfies the system suitability criteria, peak integrity, and resolution among the parent drug and its degradation products. The results indicate that the established assay method shows good selectivity and specificity suitable for stability measurements of CPF. CPF is found to be more sensitive to exposure to light and in acidic conditions, but it is stable in a basic medium. The kinetic study of the photodegradation of CPF follows an apparent first-order reaction in a variety of solvents. The solvent effects on the rates of degradation are in the decreasing order of chloroform > dichloromethane > methanol > ethanol > 2-propanol, which is irrelevant to the dielectric constant epsilon. However, the hydrogen-donating ability of the solvents is essential to the photochemical decomposition of CPF. A plot of log k versus the Kirkwood function exhibits a linear relationship in aqueous ethanolic solutions, which implies that degradation proceeds via an ionic mechanism.