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Minimum BSA to add to fractions to minimize loss upon drying

http://www.chromforum.org/viewtopic.php?t=6556

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Minimum BSA to add to fractions to minimize loss upon drying

Posted: Thu Aug 02, 2007 12:24 am
by pipettemonkey
We add BSA to our HPLC fractions (1.5mL centrifuge tube) to minimize loss of peptide to the inside of the tube during speed vac evaporation. How much is needed for this? Is the amount needed a function of the amount of liquid being evaporated?

Posted: Mon Aug 06, 2007 9:32 pm
by Jumpshooter
use 1/100 the volume of your sample extract. So if you have a 2 mL sample extract--just add 20uL of BSA. ONly small volumes of this reagent are needed--I use it when I'm stablizing isolated DNA to prepare lambda Hind3 markers with DNAP+buffer+bSA+water.
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