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Hard to get fragments

http://www.chromforum.org/viewtopic.php?t=6553

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Hard to get fragments

Posted: Wed Aug 01, 2007 2:51 pm
by ESIer
Yesterday someone accidently rebooted the MS(ESI, Triplequadrupole, Micromass,).It seems nothing wrong happened. Then I ran a biosample with LC-MS (tandam MS). I got the expected peaks with very good shape and intensity. But when I ran an authentic standard under direct injusion mode. I found the parent ions which were supposed to be fragemented very easily were really hard to be broken (I have tested that standard a couple of times). Why? I used the same method as the one I used before.

Does anyone know what is the reason?

Posted: Thu Aug 02, 2007 12:24 am
by Schmitty
Run out of Argon or other collision gas?
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