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ephedrine/pseudoephedrine separation and quantification
Posted: Fri Jul 27, 2007 8:27 pm
by ntbhat
I am looking to quantitate ephedrine/pseudoephedrine in a natural product sida cordifolia. Any ideas on the column, conditions etc. that can be used. Any suggestions will be appreciated.
Posted: Fri Jul 27, 2007 9:30 pm
by Jumpshooter
I have successfully done semiquants on differentiating methamphetamine, amphetamine, pseudophedr, and ephedrine using Thin Layer Chromatography.
ephedrine/pseudoephedrine
Posted: Fri Jul 27, 2007 11:50 pm
by ntbhat
Thanks, but I was looking to use HPLC. Any suggestions on columns, Mobile Phases etc.?
Posted: Sat Jul 28, 2007 12:15 am
by Bruce Hamilton
A detailed discussion, including methods, can be found here:-
http://rivm.nl/bibliotheek/rapporten/670220001.pdf
I'm sure there are many others available via Google...
Bruce Hamilton
Thanks, Bruce hamilton
Posted: Sat Jul 28, 2007 2:35 am
by ntbhat
Thank you for pointing me in the right direction
Posted: Sat Jul 28, 2007 4:42 am
by Schmitty
Here's another. Just replace MS/MS with DAD. You might have a problem with interfering peaks with a DAD, but you may get some good ideas.
AOAC Ephedra Submission