Chromatography Forum

By label seeker on Thursday, - 02:37 pm:

Hi.

I was wondering if any of you have knowledge about the effects of radiolabeling on chromatographic behavior. Specifically, could incorporating several 14-Carbon molecules into a small organic chemical (anthracene, for example) change the boiling point enough to give a retention time difference vs the typical 12-Carbon molecule? Specifically I'm considering a GC ststem with a typical DB-5 type column.

thanks!

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By HW Mueller on Friday, August 27, 2004 - 12:01 am:

Possibly, I had partial separation between 12C-oxalic and 13C-oxalic acid derivtives. A colleague separated deuterated from non-deuterated sugar derivatives.

You can use non radioactive material such as 13 C or D labelled molecule. Then for the identification use MS and you can show that the fragmentation and the molecular ion are not the same as a 12 C molecule.