on insulin separation

[moon704520]

do you have some ideas on insulin(gene clone produced ) separation? normally ,when it comes to insulin separation with high/medium
pressure LC,people usually use what packing media?use what mobile phase?and why?

[SIELC_Tech]

Here is example of separation for three insulins: Humalog, Humulin and Lantus:
http://www.sielc.com/compound_211.html
I am not aware about any other separation of Humalog and Humulin with LC/MS friendly conditions.
PLease download our Promix brochure for more examples:
http://www.sielc.com/Literature_Brochures.html

Contact me if ou have questions.

Vlad

[Uwe Neue]

You can find several insulin applications at the following site:

http://www.waters.com/watersdivision/pd ... tebook.htm

[Bryan Evans]

We have insulin retained on our Intrada WP-RP (widepore) column (where the larger proteins are also retained):
http://www.imtakt.com/TecInfo/TI289E.pdf

We also have insulin retained on our Cadenza HS-C18 column (where the larger proteins are excluded):
http://www.imtakt.com/TecInfo/TI290E.pdf